An Enzyme-Linked Immunosorbent Assay (ELISA) for Methylphenidate (Ritalin(R)) in Urine
نویسندگان
چکیده
منابع مشابه
The ELISA, enzyme-linked immunosorbent assay.
Featured Article: Engvall E, Perlmann P. Enzymelinked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G. Immunochemistry 1971;8:871– 4. This paper was my first as a graduate student of Professor Peter Perlmann at Stockholm University. At the time, RIAs were in full bloom, but they were too sophisticated for many areas of research and diagnosis because they required expensive e...
متن کاملDevelopment of an enzyme - linked immunosorbent assay ( ELISA ) for the
6 Department of Parasitology and Parasitic Diseases, Faculty of Veterinary Medicine, University of 7 Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Cluj-Napoca, Romania; Veterinary 8 Mycology, Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, 9 University of Liège, Liège, Belgium; Dermatology and Venereology Department, Laboratory of 10 Mycology, Centr...
متن کاملAn enzyme-linked immunosorbent assay (ELISA) for urinary free cortisol.
Since the introduction of our plasma cortisol enzyme-linked immunosorbent assay (ELISA) [l] using affinity purified antiserum [2] into the routine steroid laboratory, we have embarked on the development of a simple urinary free or unconjugated cortisol ELISA which would allow measurement of both urine and plasma cortisol on the one ELISA plate. This paper describes for the first time an ELISA f...
متن کاملImmunodiagnosis of Haemonchus contortus infection in sheep by indirect enzyme linked immunosorbent assay (ELISA)
Indirect plate enzyme-linked immunosorbent assay was standardized and evaluated for its effectivenessin immunodiagnosis of haemonchosis in experimental and clinical cases in sheep by using somatic wholeadult antigen of H. contortus. Plate ELISA was standardized using 5 μg/well antigen concentration with1:100 and 1:1000 of sera and conjugate dilution. Indirect plate ELISA was able to demonstrate...
متن کاملEnzyme immunoassay (EIA)/enzyme-linked immunosorbent assay (ELISA).
This brief note addresses the historical background of the invention of the enzyme immunoassay (EIA) and enzyme-linked immunosorbent assay (ELISA). These assays were developed independently and simultaneously by the research group of Peter Perlmann and Eva Engvall at Stockholm University in Sweden and by the research group of Anton Schuurs and Bauke van Weemen in The Netherlands. Today, fully a...
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ژورنال
عنوان ژورنال: Journal of Analytical Toxicology
سال: 2003
ISSN: 0146-4760,1945-2403
DOI: 10.1093/jat/27.6.342